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Nogueira,Paulyene Vieira; Coutinho,Givago; Pio,Rafael; Silva,Daniel Fernandes da; Zambon,Carolina Ruiz. |
ABSTRACT To support breeding programs of pear tree, on the selection of cultivars to subtropical area in Brazil, the objective of this research was to adjust the growth medium basic to pollen grain germination. The pollen grains of 'D'Água' cultivar were spread on the surface of Petri dishes containing 20 ml of culture medium in accordance with the following sequential experiments: 1) ágar (4; 6; 8 and 10 g L-1) and pH (3,5; 4,5; 5,5 and 6,5); 2) sucrose (0; 30; 60 and 90 g L-1); 3) calcium nitrate (0; 200; 400 and 800 mg L-1); 4) boric acid (0; 400; 800 and 1200 mg L-1); 5) temperature of incubation (15; 20; 25 e 30 ºC) and 6) emission time of the pollen tube (0; 1; 2; 3; 4; 5 and 6 hours after inoculation). After incubation, the germination rate of... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Pyrus communis; Pyrus pyrifolia; Breeding programs; Germination rate. |
Ano: 2016 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1806-66902016000200380 |
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Silva,Luiz Fernando de Oliveira da; Zambon,Carolina Ruiz; Pio,Rafael; Oliveira,Adelson Francisco de; Gonçalves,Emerson Dias. |
ABSTRACT Pollen grain germination in vitro indicates viability and consequently provides information related to fruit set. It also assists in the development of hybrids. Along with a suitable species, a standard culture medium is essential for evaluating pollen viability. It should contain a gelling agent consisting of carbohydrates and enhancer elements as well as have the correct pH, temperature, and incubation time. The objective of this study was to optimise the culture medium, determine the pollen germination capacity, and quantify the number of pollen grains per flower of certain olive tree cultivars. A basic sequential culture medium for pollen grain germination was determined, always utilizing the best result from the previous experiment to... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Olea europaea L.; Germination rate; Fruit set. |
Ano: 2016 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0006-87052016000100026 |
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Ranal,Marli A.; Santana,Denise Garcia de. |
In the last two centuries, papers have been published including measurements of the germination process. High diversity of mathematical expressions has made comparisons between papers and some times the interpretation of results difficult. Thus, in this paper is included a review about measurements of the germination process, with an analysis of the several mathematical expressions included in the specific literature, recovering the history, sense, and limitations of some germination measurements. Among the measurements included in this paper are the germinability, germination time, coefficient of uniformity of germination (CUG), coefficient of variation of the germination time (CVt), germination rate (mean rate, weighted mean rate, coefficient of... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Germinability; Germination rate; Germination synchrony; Germination time; Germination uniformity. |
Ano: 2006 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-84042006000100002 |
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Lopes,Rodrigo Ramos; Souza,Cleber Henrique Lopes de; Bertoncelli,Patricia; Franke,Lucia Brandão. |
ABSTRACT The objective of this study was to identify the most efficient method for overcoming coat-imposed dormancy and determine the optimal germination temperature for Ornithopus pinnatus seeds. Treatments to overcome dormancy were: intact seeds; immersion in hot water at 60 ºC, followed by soaking in the same water (unheated)/24 h; immersion in hot water at 90 ºC, followed by soaking in the same water (unheated)/24 h; mechanical scarification; chemical scarification, H2SO4/5 min; and chemical scarification, H2SO4/10 min. Percentages were calculated for germinated, abnormal, dormant, and dead seeds. Optimal germination temperatures were calculated using eight constant temperatures (0, 5, 10, 15, 20, 25, 30, and 35 °C), with 8 h of light and 16 h of... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Germination rate; Ornithopus pinnatus; Scarification; Synchronization index of germination. |
Ano: 2015 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-35982015001200413 |
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